(eBook) (PDF) Laboratory Experiments in Microbiology, 12th edition

Description

Overview

With Laboratory Experiments in Microbiology, you’ll learn basic microbiology techniques and applications through 57 class-tested exercises. This manual covers every area of microbiology for undergraduate study across diverse disciplines and is designed to help you develop essential laboratory thinking skills.

Published by Pearson (July 14th 2021) – Copyright © 2019
ISBN-13: 9780137546503
Subject: Microbiology
Category: Intro Microbiology

PART ONE MICROSCOPY

1. Use and Care of the Microscope
2. Examination of Living Microorganisms

PART TWO HANDLING BACTERIA

3. Microbes in the Environment
4. Transfer of Bacteria: Aseptic Technique

PART THREE STAINING METHODS

5. Preparation of Smears and Simple Staining
6. Negative Staining
7. Gram Staining
8. Acid-Fast Staining
9. Structural Stains (Endospore, Capsule, and Flagella)
10. Morphological Unknown

PART FOUR CULTIVATION OF BACTERIA

11. Isolation of Bacteria by Dilution Techniques
12. Special Media for Isolating Bacteria

PART FIVE MICROBIAL METABOLISM

13. Carbohydrate Catabolism
14. Fermentation
15. Protein Catabolism, Part 1
16. Protein Catabolism, Part 2
17. Respiration
18. Unknown Identification and Bergey’s Manual

PART SIX MICROBIAL GROWTH

19. Oxygen and the Growth of Bacteria
20. Determination of a Bacterial Growth Curve: The Role of Temperature
21. Biofilms

PART SEVEN CONTROL OF MICROBIAL GROWTH

22. Physical Methods of Control: Heat
23. Physical Methods of Control: Ultraviolet Radiation
24. Chemical Methods of Control: Disinfectants and Antiseptics
25. Chemical Methods of Control: Antimicrobial Drugs
26. Effectiveness of Hand Scrubbing

PART EIGHT MICROBIAL GENETICS

27. Regulation of Gene Expression
28. Isolation of Bacterial Mutants
29. Transformation of Bacteria
30. DNA Fingerprinting
31. Genetic Engineering
32. Ames Test for Detecting Possible Chemical Carcinogens

PART NINE THE MICROBIAL WORLD

33. Fungi: Yeasts and Molds
34. Phototrophs: Algae and Cyanobacteria
35. Protozoa
36. Parasitic Helminths

PART TEN VIRUSES

37. Isolation and Titration of Bacteriophages
38. Plant Viruses

PART ELEVEN INTERACTION OF MICROBE AND HOST

39. Epidemiology
40. Koch’s Postulate

PART TWELVE IMMUNOLOGY

41. Innate Immunity
42. Agglutination Reactions: Slide Agglutination
43. Agglutination Reactions: Microtiter Agglutination
44. ELISA Technique

PART THIRTEEN MICRORGANISMS AND DISEASE

45. Bacteria of the Skin
46. Bacteria of the Respiratory Tract
47. Bacteria of the Mouth
48. Bacteria of the Gastrointestinal Tract
49. Bacteria of the Urogenital Trac
50. Identification of an Unknown from a Clinical Sample
51. Rapid Identification Methods

PART FOURTEEN MICROBIOLOGY AND THE ENVIRONMENT

52. Microbes in Water: Multiple-Tube Technique
53. Microbes in Water: Membrane Filter Technique
54. Microbes in Food: Contamination
55. Microbes Used in the Production of Foods
56. Microbes in Soil: The Nitrogen and Sulfur Cycles
57. Microbes in Soil: Bioremediaton

APPENDICES.

• A. Pipetting
• B. Dilution Techniques and Calculations
• C. Use of the Spectrophotometer
• D. Graphing
• E. Use of the Dissecting Microscope
• F. Use of the Membrane Filter
• G. Electrophoresis
• H. Keys to Bacteria